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The Dakota Water Watch
Jeanne
Fromm is Water Resource Specialist
at East Dakota Water Development
District in Brookings, and
coordinates Dakota Water Watch. Jeanne
is also the newest member
of SDL&SA. Welcome
aboard, Jeanne!! The Dakota Water Watch
is a continuation of the Volunteer Monitoring Program initiated in the
early 1990’s by SDL&SA. The
Bacterial Monitoring Project, managed by Jeanne Fromm as a Pilot project
started by EDWDD AND SDL&SA
in 2007.
Background of the Volunteer Monitoring Project
President Don Marquardt, in his
Annual Update of SDL&SA in the April, 1997 “Statewide Water News”
indicated that “we plan to continue the Citizen’s Monitoring
Program…” Another article
mentions that the Citizen’s Monitoring program has been in operation for
several years (prior to ’97). Jane
Wittmeier was “Citizen Monitoring Chairperson in 1997.
Kristie Maher joined Jane in Spring, 1998, and Anne Lewis replaced
Kristie Maher later in 1998. Citizen’s Monitoring, Lake Liaison, Storm Drain Stenciling,
and Adopt-a-Stream joined into the “Special Projects Division” in
Spring, 1999, with Anne Lewis in charge working out of The Discovery
Center, Pierre. Anne
coordinated the Volunteer Monitoring Program until 2006.
Bacterial
Monitoring Project Summary
The Bacteria Monitoring Project
established 60 monitoring sites on standing and flowing waters in the
central Big Sioux watershed in seven of the eight areas indicated in the
contract. Most volunteers
monitored lakes (29 sites) and staff monitored streams and rivers. Protocols for field monitoring, sample site documentation,
sample collection and transport, culturing, and culture interpretation
were prepared prior to the field season.
The project recruited, trained, and supported 32 volunteers who
monitored 215 times between March and October of 2007.
Staff monitored 60 times.
The project
collected 295 samples for bacteriological analysis-205 from standing
waters and 90 from flowing waters. Sampling
occurred under dry-weather (84% of samples) conditions on a monthly basis
between April and October and after 1-3 storm events (16% of samples).
Field samples included 6% blanks, 8% duplicates, and 2% resamples.
Samples were delivered to three mini-labs established in Watertown,
Brookings, and Madison where 473 cultures were prepared using 3M Petrifilm™
and/or Coliscan® Easygel® methods.
The average cold-holding time was 3 hours.
The mini-labs prepared 10 laboratory blanks.
Colony forming units (CFU) per 100 mL of total coliform and Escherichia
coli (E. coli) bacteria were determined after 24 hours of
incubation at 35EC. The
project sent 51 split samples to the South Dakota State Health Lab (SDSHL)
where 144 cultures were prepared using Coliscan Eagygel-MF and other
methods. The mini-labs also
split and cultured samples to compare different methods. Roughly half (56%) of the lake cultures
contained no E. coli and most (71%) of the stream cultures exceeded
the EPA recommendation for single-sample E. coli maximum for
intense swimming use (235 CFU/100 mL).
About 11% of standing water cultures exceeded 235 CFU/100 mL.
South Dakota uses fecal coliform, not E. coli, as a
recreational water use measure. E.
coli concentrations increased in samples collected under wet-weather
conditions. All blank samples
cultured NO coliform or E. coli bacteria.
When compared to SDSHL cultures, the volunteer methods, Coliscan
Eagygel and 3M Petrifilm™, agreed that standards were exceeded 90 and 94
percent of the time, respectively.
Project data was stored in an electronic
database (EXCEL). Volunteers
mailed all field and lab datasheets to project coordinator on a monthly
basis. Ongoing review of
datasheets and database entry helped identify field method errors and data
entry mistakes. Project
updates; prepared in June, August, and October; were sent to all
volunteers and reported culture results for each sample site, provided
guidance on interpreting the results, reinforced some training elements,
and helped coordinate the project. The database was used to analyze and compare the data.
Pre- and post-project tests indicated that most volunteers learned
from the project. Ongoing
ease-of-use assessments showed that volunteers quickly found field and lab
methods easy to use. Project
results were shared with the larger community via newspaper articles and
presentations to lake groups. Participants demonstrated a commitment to the sampling
schedule and carefully followed sampling methodologies over the
seven-month field season. Volunteers
had a strong interest in information pertaining to health risks in
recreational waters. Data
analysis and intermethod comparisons suggest that both culture techniques
are good tools to “screen” for high levels of E. coli bacteria.
With some adjustments, the more “sensitive” Coliscan Easygel
method could generate statistically meaningful counts at concentrations
near the EPA recommendations for seasonal average (126 CFU/100 mL) and
single sample maximum (235 CFU/100 mL) for E. coli. Dakota Water Watch will continue to work individuals
and groups that are interested in bacteria monitoring.
New programs will be offered at several sites and existing programs
will be supported. Contact
Jeanne Fromm, 605/588-6611, for more information. *****************
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South Dakota Lakes & Streams Association
P.O. box 95
108 S. School St South Shore, SD 57263
ph. 1 (605) 756-4195 fax 1 (605) 756-4218
© 2002 all rights reserved
last updated 28 December 2007